Ding, ding, bang, ya karya wani, kuma wannan shine ɗayan kayan aikin da aka sani a cikin lab ɗin mu, gilashin gilashi.Yadda ake tsaftace gilashin gilashi da yadda ake bushewa.
Akwai abubuwa da yawa da ya kamata ku kula yayin amfani, kun sani?
- Kuse na gama gari
(I) Pipette
1. Rarrabewa: Single mark pipette (wanda ake kira babban ciki pipette), pipette mai digiri (nau'in fitarwa wanda bai cika ba, cikakken nau'in fitarwa, nau'in busawa)
- Ana amfani da pipette guda ɗaya don pipette wani nau'i na bayani daidai. Girman diamita na ɓangaren alama na pipette guda ɗaya yana da ƙananan kuma daidaito yana da girma;Pipette mai nuni yana da babban diamita kuma daidaito ya ɗan fi muni.Don haka, lokacin da ake auna adadin intiger na maganin, yawanci ana amfani da girman daidaitattun pipette guda ɗaya maimakon alamar pipette.
- Aiki:
Pipetting: don gwajin da ake buƙatar babban daidaito, goge ragowar ruwa daga tip na bututu tare da takardar tacewa, sannan kurkura ruwan ciki da waje da tip na bututu tare da ruwa mai jira har sau uku don tabbatar da cewa maida hankali na cire maganin aiki ya rage bai canza ba. Yi hankali kada a sake dawo da maganin don guje wa dilution da gurɓataccen maganin.
Lokacin da ake yin bututun maganin da za a yi amfani da shi, saka ƙarshen bututun 1-2cm a ƙasan ruwan ruwa (zurfin da yawa, bayani mai yawa yana manne da bangon waje na bututu, mai zurfi: tsotsa fanko bayan matakin ruwa ya faɗi).
Karatu: Layin gani yana kan matakin daidai da mafi ƙasƙanci na meniscus na mafita.
Saki: titin bututun ya taɓa cikin jirgin domin jirgin ya karkata kuma bututun yana tsaye.
Hagu kyauta tare da bango: Kafin a cire pipette daga kwandon karɓa, jira dakika 3 don tabbatar da cewa ruwan yana gudana gaba ɗaya.
(2) flask mai girma
Ana amfani dashi da yawa don shirya maganin daidaitaccen taro.
Kafin yin amfani da faifan volumetric, bincika ko ƙarar filayen ƙarar ya yi daidai da abin da ake buƙata;Yakamata a yi amfani da filaye masu girma dabam na Brown don shirye-shiryen abubuwa masu narkewa masu haske.Ko filogi mai niƙa ko filogin filastik yana zub da ruwa.
1. Gwajin zubar da ruwa: ƙara ruwan famfo zuwa wurin da ke kusa da layin lakabin, toshe ƙugiya da ƙarfi, danna filogi tare da yatsa, tsayawa kwalban a kife na tsawon mintuna 2, sannan a yi amfani da busasshiyar takarda tace don bincika ko akwai magudanar ruwa tare. tazarar bakin kwalbar. Idan babu ruwan yabo, juya kwalabe 180 ° kuma tsaya a kansa na wasu mintuna 2 don dubawa.
2. Bayanan kula:
Dole ne a yi amfani da sandunan gilashi lokacin canja wurin mafita zuwa filaye masu girma;
Kada ku riƙe kwalban a tafin hannun ku don guje wa faɗaɗa ruwa;
Lokacin da ƙarar da ke cikin ƙwanƙolin ƙarar ya kai kusan 3/4, girgiza kwalban volumetric sau da yawa (kada ku juyo), don sa maganin ya haɗu da kyau.Sa'an nan kuma sanya kwalban volumetric a kan tebur kuma a hankali ƙara ruwa har sai ya kasance kusa da layin 1cm, jira na minti 1-2 don barin bayani mai ma'ana a bangon kwalban.Ƙara ruwa zuwa mafi ƙasƙanci a ƙasa da matakin ruwa mai lanƙwasa da tangent zuwa alamar;
Maganin zafi ya kamata a sanyaya zuwa zafin jiki na dakin kafin a yi masa allura a cikin filastar volumetric, in ba haka ba ana iya haifar da kuskuren girma.
kwalban volumeter ba zai iya ɗaukar maganin na dogon lokaci ba, musamman ma lye, wanda zai lalata gilashin kuma ya sa ƙugiya ya tsaya kuma ya kasa buɗewa;
Lokacin da aka yi amfani da kwalban volumetric, kurkura shi da ruwa.
Idan ba a daɗe ana amfani da shi ba, a wanke a goge shi a bushe sannan a kwaba shi da takarda.
- Hanyar wankewa
Ko kowane nau'in gilashin da aka yi amfani da su a cikin dakin gwaje-gwaje na jiki da na sinadarai suna da tsabta sau da yawa yana rinjayar aminci da daidaito na sakamakon bincike, don haka yana da matukar muhimmanci a tabbatar da cewa gilashin da aka yi amfani da shi yana da tsabta.
Akwai hanyoyi da yawa don wanke gilashin gilashi, wanda ya kamata a zaba bisa ga buƙatun gwajin, yanayin datti da digiri na ƙazanta.Na'urar aunawa wanda ke buƙatar auna maganin daidai, ba shi da sauƙi don amfani da goga lokacin tsaftacewa, saboda ana amfani da goga na dogon lokaci, yana da sauƙin sa bangon ciki na na'urar aunawa, da kayan da za su kasance. auna ba daidai ba ne.
Binciken tsaftar gilashin gilashi: bangon ciki ya kamata a jika gabaɗaya da ruwa ba tare da beads ba.
Hanyar tsaftacewa:
(1) Goga da ruwa;
(2) Yin wanka tare da maganin sabulu ko sabulu (wannan hanyar ba a ba da shawarar ba don chromatography ko gwaje-gwajen spectrometry na taro, surfactants ba su da sauƙin tsaftacewa, wanda zai iya rinjayar sakamakon gwaji);
(3) Yi amfani da ruwan shafawa na chromium (20g potassium dichromate yana narkar da shi a cikin 40g mai zafi da ruwa mai gauraya, sannan kuma a hankali ƙara 360g na masana'antu concentrated hydrochloric acid): yana da ƙarfi mai ƙarfi don cire mai daga kwayoyin halitta, amma yana da lalata sosai kuma yana da. wasu guba.Kula da aminci;
(4) Sauran kayan shafawa;
Maganin alkaline potassium permanganate: 4g potassium permanganate ana narkar da shi a cikin ruwa, ana zuba 10g potassium hydroxide kuma a dill da ruwa zuwa 100ml.Ana amfani da shi don tsaftace tabon mai ko wasu abubuwan halitta.
Oxalic acid ruwan shafa: 5-10g oxalic acid ana narkar da shi a cikin ruwa 100ml, kuma an ƙara ɗan ƙaramin adadin hydrochloric acid.Ana amfani da wannan maganin don wanke manganese dioxide da aka samar bayan wankewar potassium permanganate.
Iodine-potassium iodide ruwan shafa fuska (1g aidin da 2g potassium iodide ana narkar da su a cikin ruwa kuma a diluted da ruwa zuwa 100ml): ana amfani da shi don wanke datti mai duhu mai launin ruwan kasa na azurfa nitrate.
Maganin pickling mai tsabta: 1: 1 hydrochloric acid ko nitric acid.Ana amfani da su don cire ions masu ganowa.
Maganin alkaline: 10% sodium hydroxide maganin ruwa mai ruwa.Sakamakon raguwa ta hanyar dumama ya fi kyau.
Abubuwan kaushi na halitta (ether, ethanol, benzene, acetone): Ana amfani da su don wanke tabon mai ko abubuwan da aka narkar da su a cikin sauran ƙarfi.
3. Drying
Ya kamata a wanke kayan gilashin kuma a bushe don amfani daga baya bayan kowace gwaji.Gwaje-gwaje daban-daban suna da buƙatu daban-daban don matakin bushewar kayan gilashi.Misali, flask triangular da aka yi amfani da shi don titrating acidity ana iya amfani da shi bayan an wanke, yayin da flask ɗin triangular da ake amfani da shi wajen tantance kitse yana buƙatar bushewa.Ya kamata a bushe kayan aiki bisa ga buƙatu daban-daban.
(1) Busasshen iska: idan ba ku buƙatar shi cikin gaggawa, ana iya bushe shi a juye;
(2) bushewa: Ana iya bushe shi a cikin tanda a 105-120 ℃ (na'urar aunawa ba za a iya bushewa a cikin tanda ba);
(3) bushewa: Ana iya amfani da iska mai zafi don bushewa cikin gaggawa (na'urar bushewa ta gilashi).
Tabbas, idan kuna son hanyar tsaftacewa da bushewa mai aminci kuma mai inganci, zaku iya zaɓar na'urar wanke kayan gilashin dakin gwaje-gwaje ta XPZ.Ba zai iya tabbatar da tasirin tsaftacewa kawai ba, amma kuma yana adana lokaci, ƙoƙari, ruwa da aiki.Wurin wanki na gilashin dakin gwaje-gwaje da XPZ ya samar yana ɗaukar sabuwar fasahar tsaftacewa ta duniya.Yana iya kammala tsaftacewa ta atomatik, disinfection da bushewa tare da maɓalli ɗaya, yana kawo muku sabon ƙwarewar inganci, sauri da aminci.Haɗuwa da tsaftacewa da bushewa ba wai kawai inganta matakin da ingancin gwaji na atomatik ba, amma kuma yana rage yawan gurɓataccen gurɓataccen abu da lalacewa a lokacin aiki.
Lokacin aikawa: Agusta-06-2020