Anti-crossions, ingantaccen gwajin DNA na iya bayyana gaskiya a ɓoyayyun sasanninta

Anti-cross-contamination86

A yawancin fina-finai da ayyukan adabi, dakunan gwaje-gwaje na bincike sun bayyana a matsayin rayuwa ta musamman kuma mai mahimmanci, musamman ma shirin gwajin gano DNA yakan zama mabuɗin samun alamu da warware lamura.To sai dai idan ana shakku kan sahihancin sakamakon gwajin da aka gabatar, to a zahiri ba zai zama hujjar shari'a ba, balle a bayyana gaskiya a boye.Akwai wani abu na musamman na jiki da sinadarai wanda dakunan gwaje-gwaje na bincike ya kamata su bi da su cikin taka tsantsan, wato don hana samfuran DNA da za a gwada su gurɓata a cikin dakin gwaje-gwaje.A halin yanzu, akwai dalilai daban-daban na gurɓatar DNA.Daga cikin su, yiwuwar kamuwa da cutar giciye shine mafi girma.
Anti-cross-contamination781

Kamar sauran dakunan gwaje-gwaje, kayan da ake amfani da su da kayan aiki a cikin dakunan gwaje-gwaje na bincike sun gurɓata a lokuta da yawa.Musamman ma, kamuwa da cuta tsakanin samfuran DNA dangane da halayen PCR, sauran kayan gwaji da shaida ta zahiri, da masu gwajin kansu sune mafi wahalar ganowa.Waɗannan gurɓatattun gurɓatattun abubuwa sun haɗa da ƙwayoyin halitta, jini, kyallen takarda, da kuma kayan aikin gwaji, kayan wanke-wanke da sauran ƙazanta.

Yana da kyau a jaddada cewa gilashin da za a sake amfani da su a cikin dakunan gwaje-gwaje na bincike kamar kwantena samfurin, kwalabe na reagent, bututun gwaji, pipettes, flasks, jita-jita na petri, da dai sauransu. Rashin isassun aiwatar da su, rashin bin ka'idoji, da rashin bin ka'idodin aikin wankewa suna ɗaya daga cikin. masu laifi waɗanda ke haifar da gano kuskure da ƙarshe na bincike.

Anti-cross-contamination1580

Irin wannan gurɓataccen gilashin gilashin wata barazana ce a fili ga sakamakon gwajin, don haka menene mabuɗin warware shi?

Da farko, lokacin da aka sami wani yanayi da ake zargi da lalata DNA, yakamata a sake duba sakamakon gwajin cikin lokaci don dawo da kuskuren.Wannan shine fifiko na farko.

Sa'an nan kuma, gudanar da binciken kula da inganci akan abubuwan da ake amfani da su na gwaji, gami da kwantena gilashi, reagents, da sauransu, don ƙara tabbatar da tushen gurɓatawa.

A kan wannan, inganta hanyoyin tsaftacewa na gilashin gilashi don gyara kurakurai, don kauce wa kuskuren kuskure daga sake faruwa.

Na uku, kawai ta hanyar ƙarfafa gaba ɗaya matakan hana gurɓatawa da ƙazantawa na dakin gwaje-gwaje don samar da tsarin gudanarwa na hukuma zai iya inganta tsarin tsaftacewa ya zama mai ma'ana da tasiri.

A haƙiƙa, ƙwararren ɗakin binciken bincike ya kamata ya kasance yana da keɓancewa da yanki mai zaman kansa don gwajin DNA don tabbatar da cewa an rage gurɓata gurɓata a cikin hanyoyin gwaji daban-daban.Misali, karban shari'ar da wurin ajiyar samfurin, wurin cirewar DNA, yankin haɓaka DNA, yankin gano DNA, yankin bincike na farko, yankin bincike na sakamako, yanki na shirye-shirye, yankin haɓaka DNA, wurin ganowa, da sauransu.Daga cikin su, tsaftace gilashin gilashi a cikin yanki na shirye-shiryen zai taimaka wajen kara yawan yiwuwar rashin nasarar sakamakon gwajin.

Anti-cross-contamination2954

Ya kamata a lura cewa yawancin dakunan gwaje-gwaje na ƙwararru, gami da dakunan gwaje-gwaje na bincike, har yanzu suna amfani da hanyoyin tsabtace hannu marasa inganci don magance matsalar gurɓataccen gurɓataccen kayan gilashi.Amma wannan hanyar ba ta inganta ainihin haɗarin ɗan adam na kamuwa da cuta ba.

Abin da ya fi haka, rashin amfanin tsabtace kayan gilashin hannu ya wuce wannan.

MTsaftace kayan gilashi na shekara-shekara ba wai kawai kasawa sosai don tsaftace kayan aikin dakin gwaje-gwaje ba kuma zai shafi ƙarshen gwajin DNA da ganowa, zai kuma haifar da jerin saɓani kamar ɓarna albarkatu, rikitarwar ayyukan tsaftacewa, da haɗarin aminci ga ma'aikatan dakin gwaje-gwaje. .A wannan lokacin, ana amfani da maganin rigakafi atomatik gilashin wankiamfani da su a dakunan gwaje-gwaje na bincike na kasa da kasa zai taimaka wajen inganta wannan yanayin.

Anti-cross-contamination3773

Laboratory gilashin wankizai iya tsaftace nau'ikan gilashin gwaje-gwaje iri-iri daidai a cikin aminci, tsari, da hanya mai hankali don sanya shi ya dace da ƙa'idodin GMP da FDA.Idan aka kwatanta da hanyar tsaftace hannu, da Wutar Lantarkizai iya bin tsarin tsaftacewa a cikin dukan tsari, wanda ya dace don samun bayanan bayanai masu mahimmanci a bangarori daban-daban.Waɗannan bayanan suna da ma'ana mai girma don kau da saura yanayi gami da gurɓatar da gwajin DNA ke fuskanta.Musamman idan akwai bambance-bambance da shakku game da sakamakon gwajin!

Tare da ci gaba da haɓaka fasahohin da suka dace, dakunan gwaje-gwaje na bincike za su ɗauki ƙarin nauyi a cikin aiwatar da shari'ar.Ta wannan hanyar, buƙatun kowane sakamakon gwajin dakin gwaje-gwaje da daidaitawa da daidaito a zahiri za su ci gaba da ƙaruwa.Hanyoyin da suka haɗa da gwajin DNA na iya ba da garantin sakamako mai tsabta kawai da samun daidaitaccen ƙarshe da shaida idan sun yi nasara wajen lalata.Wannan wani abu ne da ya kamata kowane dakin gwaje-gwaje ya kamata ya tuna.


Lokacin aikawa: Afrilu-02-2021